What is the Wolverine peptide blend?
Wolverine is a two-component research blend supplied in a single lyophilized vial: 10MG BPC-157 and 10MG TB-500. Each component has its own published literature, almost all of it from cell culture and rodent models, and only one published experiment has tested the two together. This guide summarizes what has been reported for each component, states the study model for every finding, and describes that one combined study. Disguised Alpha supplies Wolverine for laboratory research use only. Nothing on this page is guidance for use in people.
Wolverine composition
| Component | Per vial | What it is |
|---|---|---|
| BPC-157 | 10MG | Synthetic 15-residue peptide, a partial sequence of a protein described in human gastric juice |
| TB-500 | 10MG | Name used for an N-terminally acetylated fragment of thymosin beta-4 (Ac-LKKTETQ) |
| Form | 20MG total | Lyophilized powder; third-party tested, with the certificate for each lot published on the product page |
Amounts are from the Wolverine product page. A blend has no single CAS number or molecular weight: its identity is defined by its components and by the lot certificate.
What the research covers, component by component
BPC-157
BPC stands for body protection compound, the name its discoverers gave to a protein isolated from human gastric juice; BPC-157 is a partial sequence of that protein (Chang et al., 2011). Most of its literature comes from the discovering group. In rat gastric mucosa the peptide was studied alongside agents that block or supply nitric oxide (Sikirić et al., 1997), and a four-week rat model of short bowel syndrome tracked body weight, villus height and anastomosis strength in BPC-157 groups (Sever et al., 2009).
A separate group reported increased outgrowth from rat tendon explants and higher fibroblast survival and migration, with activation of the FAK-paxillin pathway (Chang et al., 2011). A 2025 systematic review of the musculoskeletal literature counted 35 preclinical studies and one clinical study, and noted that BPC-157 has no FDA approval (Vasireddi et al., 2025). The BPC-157 research guide covers it in full.
TB-500 and thymosin beta-4
Analytical work on material sold as TB-500 identified the acetylated 17 to 23 fragment of thymosin beta-4, Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln, and confirmed it by independent synthesis (Esposito et al., 2012). A doping-control method study described the same fragment as a synthetic version of an active region of the parent protein (Ho et al., 2012).
Most biological data concern the full 43-residue protein: in a rat full-thickness wound model thymosin beta-4 increased re-epithelialization and collagen deposition and stimulated keratinocyte migration in culture (Malinda et al., 1999), and in the mouse heart it formed a complex with PINCH and integrin-linked kinase that activates Akt (Bock-Marquette et al., 2004). Results for the full protein should not be assumed to carry over to the fragment.
The one study that tested both
A single published experiment compared four groups in one rat Achilles transection and repair model: control, BPC-157, TB-500 and the two combined. At four weeks it reported a significant biomechanical advantage in the TB-500 group and no additive effect from the combination (Biçer et al., 2026).
That is the whole of the published evidence on the pair. There are no data on how the two interact in solution or on stability of the combined material, so studies using Wolverine should handle it as a defined mixture and include controls for each component. The BPC-157 vs TB-500 comparison sets the two side by side.
Key studies
- Chang CH, et al. J Appl Physiol. 2011. PMID 21030672. Rat tendon explants and cultured tendon fibroblasts.
- Sikirić P, et al. Eur J Pharmacol. 1997. PMID 9298922. Rat gastric mucosa and nitric oxide pathway agents.
- Sever M, et al. Dig Dis Sci. 2009. PMID 19093208. Rat model of short bowel syndrome.
- Vasireddi N, et al. HSS J. 2025. PMID 40756949. Systematic review of the musculoskeletal literature.
- Esposito S, et al. Drug Test Anal. 2012. PMID 22962027. Synthesis and analytical identification of the acetylated 17 to 23 fragment.
- Ho EN, et al. J Chromatogr A. 2012. PMID 23084823. Doping-control detection method for the fragment.
- Malinda KM, et al. J Invest Dermatol. 1999. PMID 10469335. Rat full-thickness wound model and keratinocyte migration assay (full thymosin beta-4).
- Biçer O, et al. Jt Dis Relat Surg. 2026. PMID 42542926. Rat Achilles repair model: BPC-157, TB-500 and the combination.
Handling and storage of lyophilized Wolverine
General laboratory practice for this material:
- Store the lyophilized powder frozen (about -20°C) and protected from light until use, as for its individual components.
- Let the sealed vial reach room temperature before opening so moisture does not condense on the powder.
- Reconstitute in a sterile solvent or buffer suited to the assay, adding it gently down the vial wall and swirling rather than vortexing.
- After reconstitution, keep solutions at 2 to 8°C and protected from light, and split stock into single-use aliquots to avoid freeze/thaw cycles.
- Record the lot number and keep its certificate with your notes.
Frequently asked questions
What is the Wolverine peptide blend?
A research blend of two peptides in one vial: BPC-157 (10MG) and TB-500 (10MG).
Is there published research on BPC-157 and TB-500 together?
One study. In a rat Achilles repair model, the combination showed no additive effect over TB-500 alone at four weeks (Biçer et al., 2026).
Wolverine vs KLOW vs GLOW: what is the difference?
Wolverine holds BPC-157 and TB-500. GLOW adds GHK-Cu, and KLOW adds both GHK-Cu and KPV. The KLOW research guide covers the four-component blend.
Is TB-500 the same as thymosin beta-4?
Not exactly. Published analysis of material sold as TB-500 identified a seven-residue acetylated fragment of thymosin beta-4 rather than the full 43-residue protein (Esposito et al., 2012). Identity for each lot is on its certificate.
Where can researchers buy the Wolverine blend?
Disguised Alpha sells it for laboratory research, third-party tested with the certificate published on the product page: Wolverine (BPC-157 / TB-500).
Related compounds and guides
- BPC-157 research guide
- BPC-157 vs TB-500 comparison
- KLOW research guide
- GLOW (GHK-Cu / BPC-157 / TB-500)
- COA portal: every published certificate, by lot
This product is for research use only. It has not been evaluated for safety or effectiveness in humans. Not for human consumption. All products are intended for laboratory research purposes only.